Day 4 of growing Pleurotus ostreatus cultures from spore. Only one plate got contaminated, but it was bad. There are two contaminant fungi going to battle, and around three possible bacterial colonies. I must have been losing it at the end, haha! Can’t wait to see how they progress!
I went and skimmed through that whole series, and then I found another video where he discusses the problems of going spore to grain, and even assuming I did get some viable mycelium on these plates, I’m thinking it’s going to be nearly impossible to select them properly. I suppose I could just rinse and repeat into new plates, but then I won’t know if it’s a viable diploid/dikaryon or not.
Yet, the video I initially watched https://www.youtube.com/watch?v=tYK0hLeanVc demonstrated just grabbing some spores with a sterile blade and wiping that across agar. So maybe it is a reasonable method? One thing’s for sure, there’s a lot of colonies along the inoculation line, and it’s already probably too late to start isolating like you would with the method demonstrated by Fresh From the Farm.