Researchers have successfully realized logic gates using DNA crystal engineering, a monumental step forward in DNA computation. Their findings were published in Advanced Materials. Using DNA double crossover-like motifs as building blocks, they constructed complex 3D crystal architectures. The logic gates were implemented in large ensembles of these 3D DNA crystals, and the outputs were visible through the formation of macroscopic crystals. This advancement could pave the way for DNA-based biosensors, offering easy readouts for various applications. The study demonstrates the power of DNA computing, capable of executing massively parallel information processing at a molecular level, while maintaining compatibility with biological systems.

Journal Article

Implementing Logic Gates by DNA Crystal Engineering

Abstract:

DNA self-assembly computation is attractive for its potential to perform massively parallel information processing at the molecular level while at the same time maintaining its natural biocompatibility. It has been extensively studied at the individual molecule level, but not as much as ensembles in 3D. Here, the feasibility of implementing logic gates, the basic computation operations, in large ensembles: macroscopic, engineered 3D DNA crystals is demonstrated. The building blocks are the recently developed DNA double crossover-like (DXL) motifs. They can associate with each other via sticky-end cohesion. Common logic gates are realized by encoding the inputs within the sticky ends of the motifs. The outputs are demonstrated through the formation of macroscopic crystals that can be easily observed. This study points to a new direction of construction of complex 3D crystal architectures and DNA-based biosensors with easy readouts.