It’s a wav file in Jellyfin. I have made sure the file in question has not been corrupted. I’ve also confirmed it’s not a Jellyfin issue as I’ve tested and confirmed that it works in chromium. Every other wav file (or otherwise) works perfectly fine. If it matters, I am using the Arkenfox user.js
Song name: Coming Up Milhouse by Dankmus
Interesting. Unfortunately I’m away and unable to test the file on my own Jellyfin server. But it is a relatively high quality wav file, if for some reason Jellyfin isn’t transcoding it, it’s big enough to potentially cause that studdering just trying to stream that much data that fast (although that could also potentially happen if Jellyfin is transcoding it on the fly and your server hardware can’t keep up).
One suggestion I could make would be to try to convert the file to flac manually, and see if that makes a difference. You shouldn’t lose any quality doing that, and between the smaller file size and different format Jellyfin might treat it differently enough that it could work.