So, is it literally just repeatedly creating the necessary conditions and hoping the stuff will react the right way? Or is it a strict process that needs to be done just so or it’ll ruin the whole thing? Or both?
Biochem is incredibly sensitive to seemingly minor changes in conditions or procedure. A former coworker of mine had to change careers after the procedure she had to follow to run the assays for her master’s thesis gave her severe RSI. She couldn’t alter the procedure for ergonomics, though, because even something like changing the angle that she held the pipettor at could throw off the results.
In biopharma work, it’s not at all uncommon when trying to manufacture a biologic to find a process that works reliably in the lab but doesn’t give the same results when scaled up to production-size bioreactors, such that there’s often a whole stage of R&D devoted to taking a procedure from the lab and reproducing it on successively larger pieces of equipment, while working out all the tweaks and adjustments needed to make things work and optimize production.
Alas, my knowledge on the topic is limited – I work as a lab planner, and what I wrote above is most of what I’ve gleaned over the years of designing process development and scale-up labs. Past a point I just ooh and aah appreciatively at the big robotized bioreactor arrays my clients are putting in. Hopefully someone with a deeper background can point you in the right direction!
Yeah, kind of a little bit of both. Assuming that this is about bacterial transformation, it kind of goes like this in the lab. It’s from 2012 so there are probably easier techniques with fewer steps.
So, is it literally just repeatedly creating the necessary conditions and hoping the stuff will react the right way? Or is it a strict process that needs to be done just so or it’ll ruin the whole thing? Or both?
Biochem is incredibly sensitive to seemingly minor changes in conditions or procedure. A former coworker of mine had to change careers after the procedure she had to follow to run the assays for her master’s thesis gave her severe RSI. She couldn’t alter the procedure for ergonomics, though, because even something like changing the angle that she held the pipettor at could throw off the results.
In biopharma work, it’s not at all uncommon when trying to manufacture a biologic to find a process that works reliably in the lab but doesn’t give the same results when scaled up to production-size bioreactors, such that there’s often a whole stage of R&D devoted to taking a procedure from the lab and reproducing it on successively larger pieces of equipment, while working out all the tweaks and adjustments needed to make things work and optimize production.
Fascinating. Do you know any good resources where I can learn more details like this? I’m doing undergrad biochem rn…
Alas, my knowledge on the topic is limited – I work as a lab planner, and what I wrote above is most of what I’ve gleaned over the years of designing process development and scale-up labs. Past a point I just ooh and aah appreciatively at the big robotized bioreactor arrays my clients are putting in. Hopefully someone with a deeper background can point you in the right direction!
Yeah, kind of a little bit of both. Assuming that this is about bacterial transformation, it kind of goes like this in the lab. It’s from 2012 so there are probably easier techniques with fewer steps.
And these (11:00-16:00) are the basics about bacterial transformation.